Mobile Conveyor Belts, AI, ML and Microscopes
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How our platforms identify micro-organisms and pollution.

How can companies claim to eliminate pathogens when they don't know what they are eliminating?

With a large amount of useful data obtained, the identity of airborne and surface micro-organisms becomes more accurate.  The larger the pool of data, more data for testing which leads to accurate identification of what types of airborne and surface microbes are present. This is the conveyor belt microbe identification application. Thousands of tests from thousands of scans is the only way to get an accurate understanding as to what is lurking on surfaces and in the air. 

Drones and robots using glass plates (microscope slides) while maintaining a static electricity charge attract airborne and surface microbes for testing. The plates are fed onto a conveyor belt by the drones and robots whereas thousands of readings can take place in a minimal amount of time in a 10,000 cubic foot space .  This application is mobile and can be used in large indoor structures and becomes more accurate with an increase in the number of drones, robots and conveyor belts in operation. Artificial Intelligence algorithms manage the entire system while Machine Learning algorithms learn from every scan.

How the platform eliminates microbes and creates a biofilm that negates microbe buildup.

 

The application through machine learning algorithms learns from prior scans, collects that data, organizes that data, processes that data and gives scenarios as to what may be up and coming -AI projections. By obtaining data and data points from surface and airborne microbes and matter, our artificial intelligence platform decides what application and or behavior must eliminated and or modified and what application is needed to eliminate the threat to people, plants and animals.   One of our applications administered by our drones and robots is the application of  rhamnolipid biosurfactants .  Rhamnolipid can break apart the cell walls of some pathogens and after doing so, creates a biofilm to deter the buildup of microbes. 

What is a  Biosurfactant

biosurfactant  is a surfactant (synthetic- made made soap) that is a chemical secreted from bacteria.  
Rhamnolipid is known as a biosurfactant and is secreted from the bacterium -  pseudomonas aeruginosa .  Surfactants are soaps made from petroleum.  Biosurfactants  are soaps made without petroleum and are ubiquitous. They are found indoors and outdoors.  They can be found on tabletops, clothes, trees and animals.  Rhamnolipid (with rhamnolipid being both singular and plural i.e. fish) have widespread application and their utility across several diverse industries (pharmaceutical, cosmetics, food, environmental clean-up, agriculture, water treatment, oil and heavy petroleum recovery (enhanced oil recovery) and soil washing) is being recognized world wide as a non-toxic, biodegradable environmentally safe application.  Although, rhamnolipid have been studied for more than sixty years (discovered in 1949), interest in rhamnolipids has increased since the 1990’s as the search for more ecological responsible products intensified.

Concurrently, researchers have made great progress in the successful application of rhamnolipid in the agricultural and medical industries. The founder has developed a cost efficient process for large scale rhamnolipid production.

Recently, their utility as a biological pesticide has been gaining attention.  Traditional synthetic pesticides have a long history of being extremely toxic, persistent, non-specific and harmful to the environment while leaving a negative carbon imprint on the environment.  Severe pollution and ecological problems have been associated with the production and use of pesticides.  Metabolic products and degradation residuals have proven to be long-term environmental pollutants, especially when the compounds contained toxic metals, chlorine, and fluorine.  Most of these have been banned or are limited to very restrictive use.

In contrast, rhamnolipids are naturally occurring compounds found in soils, water, oil spills, and on plants.  They are produced and purified using natural “green” technology.  Although they are stable over a wide range of temperatures and pressures, they are naturally biodegradable.  Rhamnolipid contains only the common elements of carbon, hydrogen, and oxygen.  During biodegradation rhamnolipids are broken down into rhamnose sugars and common fatty acids that are easily metabolized.  Their potential as a natural “green” biological pesticide to replace the use of chemical pesticides is far reaching.

Biosurfactant Technology

Rhamnolipids are Biosurfactants and also known as Surfactants.  A Surfactant is derived from the word “Surface active agent”.

Biosurfactants are powerful chemicals created by nature that are non-toxic.  The name is derived from  BIO (related to life or living as in a living organism)  and Surface Active Agent —(SURFACe acTive AgeNTs -----  Biosurfactants ) is a surface-active agent such as a detergent like soap. Water normally cannot dissolve oil and grease to remove them from surfaces, but surfactant molecules surround oil and grease molecules, holding them in the water so they can be washed away.      

Loving and hating ends - polar opposites

Each surfactant molecule has a hydrophilic (water-loving) head that is attracted to water molecules AND a hydrophobic (water-hating) tail that repels water and simultaneously attaches itself to the crude oil.  These opposing forces loosen oil and suspend it in the water.

The bacteria, pseudomonas aeruginosa is everywhere. It can be found indoors on furniture, outdoors in aquatic environments, at the base of trees and, practically, everywhere else. It is literally everywhere. Rhamnolipid is a chemical produced from a bug called pseudomonas aeruginosa  and  are produced by a fermentation process similar to those used to produce fermented products such as beer and yogurt.

The bug is added to a fermentation tank and provided a nutrient source, and under properly controlled conditions the result will be rhamnolipid. The rhamnolipid are extracted, processed to remove any residual bacteria, purified, and the resultant mixture diluted into a final product. The amount and mixture of rhamnolipid material in the final solution can be precisely controlled.

Biosurfactants vs Synthetic Surfactants.

Synthetic surfactants are man-made and mostly derived from petroleum “oil based” products. They have been widely used in industry for many years because they work and were relatively inexpensive.  Synthetic surfactants have serious ecological impacts, both by depleting a non-renewal resource and by leaving non-biodegradable and harmful byproducts.

Rhamnolipid Registrations by the Founder

United States Environmental Protection Agency “EPA”
Rhamnolipid, Inc. developed RhamnoWash 10™, a naturally derived Biosurfactant, consisting of lipid and rhamnose sugar molecules.  In 2015, the U.S. Environmental Protection Agency (EPA) issued a letter regarding the listing of RhamnoWash 10™ as a Surface Washing Agent on National Oil and Hazardous Substance Pollution Contingency Plan (NCP) Product Schedule maintained by the USEPA.  This schedule lists the various products that can be used during spill response to oil discharges.  In order to list RhamnoWash 10™, Rhamnolipid, Inc. submitted technical product data to the USEPA pursuant to 40 CFR 300.915(b).

Florida Department of Environmental Protection
In 2014, the Florida Department of Environmental Protection “FDEP” granted approval for rhamnolipid wastewater and soil washing applications.  The letter indicates that the Division of Waste Management accepts Rhamnolipid Aqueous Solution for in situ bioremediation of petroleum hydrocarbons and metals in groundwater and soil.